Review



bs 0961r  (Bioss)


Bioz Verified Symbol Bioss is a verified supplier
Bioz Manufacturer Symbol Bioss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Bioss bs 0961r
    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
    Bs 0961r, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody/pmc11050135-18-9-6
    Average 94 stars, based on 9 article reviews
    bs 0961r - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells"

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25084147

    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
    Figure Legend Snippet: List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).

    Techniques Used: Staining

    Related Articles

    Staining:

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells
    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.



    Similar Products

    bs 0961r  (Bioss)
    94
    Bioss bs 0961r
    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
    Bs 0961r, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody/pmc11050135-18-9-6
    Average 94 stars, based on 1 article reviews
    bs 0961r - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti leptin receptor primary antibody  (Bioss)
    94
    Bioss anti leptin receptor primary antibody
    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
    Anti Leptin Receptor Primary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody/pmc12914310-117-21-26
    Average 94 stars, based on 1 article reviews
    anti leptin receptor primary antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti lepr  (Bioss)
    94
    Bioss anti lepr
    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
    Anti Lepr, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody/pm39002837-136-40-42
    Average 94 stars, based on 1 article reviews
    anti lepr - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    leptin receptor polyclonal antibody  (Bioss)
    94
    Bioss leptin receptor polyclonal antibody
    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
    Leptin Receptor Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody/custom%40bs-0961r%4038673732
    Average 94 stars, based on 1 article reviews
    leptin receptor polyclonal antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    leptin receptor rabbit polyclonal antibody  (Bioss)
    94
    Bioss leptin receptor rabbit polyclonal antibody
    Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, <t>leptin,</t> or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.
    Leptin Receptor Rabbit Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody/pmc11050135-18-0-6
    Average 94 stars, based on 1 article reviews
    leptin receptor rabbit polyclonal antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    lepr  (Bioss)
    92
    Bioss lepr
    Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, <t>leptin,</t> or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.
    Lepr, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody%2C+PE+Conjugated/pm35403694-53-9-11
    Average 92 stars, based on 1 article reviews
    lepr - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier
    bs 0961r pe rrid ab 11051477 rat anti mouse b220 cd45r apc cy7  (Bioss)
    92
    Bioss bs 0961r pe rrid ab 11051477 rat anti mouse b220 cd45r apc cy7
    Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, <t>leptin,</t> or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.
    Bs 0961r Pe Rrid Ab 11051477 Rat Anti Mouse B220 Cd45r Apc Cy7, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+0961r/Leptin+receptor+Polyclonal+Antibody%2C+PE+Conjugated/pmc08635308__41467_2021_27263_MOESM3_ESM-30-96-93
    Average 92 stars, based on 1 article reviews
    bs 0961r pe rrid ab 11051477 rat anti mouse b220 cd45r apc cy7 - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Staining

    Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: MTT Assay, Control, Standard Deviation

    Immunofluorescence staining of leptin receptor in BME-UV1 bovine mammary epithelial cells. Panels I and II present the localization of leptin receptor detected using primary antibodies (cat. no. bs-0961; Bioss Antibodies), followed by secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); nuclei were counterstained with 7-amino actinomycin (7-AAD, red fluorescence). Panel III presents no primary antibody control. Micrographs were taken at 600× magnification. Scale bar: 20 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: Immunofluorescence staining of leptin receptor in BME-UV1 bovine mammary epithelial cells. Panels I and II present the localization of leptin receptor detected using primary antibodies (cat. no. bs-0961; Bioss Antibodies), followed by secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); nuclei were counterstained with 7-amino actinomycin (7-AAD, red fluorescence). Panel III presents no primary antibody control. Micrographs were taken at 600× magnification. Scale bar: 20 μm.

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Immunofluorescence, Staining, Fluorescence, Control

    Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. ( A ) Representative dot-plots of Annexin V/PI double staining in untreated control cells; ( B ) graph showing percentage of apoptotic cells (sum of Annexin V pos /PI neg and Annexin V pos /Pi pos cells) in control and adipokine-treated cells; ( C ) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; ( D , E ) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. ( A ) Representative dot-plots of Annexin V/PI double staining in untreated control cells; ( B ) graph showing percentage of apoptotic cells (sum of Annexin V pos /PI neg and Annexin V pos /Pi pos cells) in control and adipokine-treated cells; ( C ) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; ( D , E ) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Double Staining, Control, Western Blot, Standard Deviation

    Concentration of αS1-casein in BME-UV1 cells ( A ) and media ( B ) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Untreated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance ( p < 0.05).

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: Concentration of αS1-casein in BME-UV1 cells ( A ) and media ( B ) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Untreated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance ( p < 0.05).

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Concentration Assay, Negative Control, Positive Control, Standard Deviation, Comparison

    Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 μm.

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Immunofluorescence, Staining, Cell Culture, Control, Fluorescence

    Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software ( https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software ( https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Cell Culture, Control, Software, Standard Deviation, Microscopy

    List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).

    Journal: International Journal of Molecular Sciences

    Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

    doi: 10.3390/ijms25084147

    Figure Lengend Snippet: List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).

    Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

    Techniques: Staining